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Phenotypic characterization of NKT-like cells and evaluation of specifically related cytokines for the prediction of unexplained recurrent miscarriage

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dc.contributor.authorKhalaf, Wafaa S.
dc.contributor.authorMahmoud, Mohammad R.A.
dc.contributor.authorHashem, Hany R.
dc.contributor.authorSoliman, Wafaa E.
dc.contributor.authorElkhatib, Walid F.
dc.date.accessioned2023-02-02T10:55:54Z
dc.date.available2023-02-02T10:55:54Z
dc.date.issued2021-11-15
dc.description.abstractProblem Immune system dysregulation is a major cause of unexplained recurrent miscarriage (URM). Women with URM need screening for their pregnancy microenvironment and immune regulators, to prevent spontaneous abortion. Method of study In this study, we evaluated NKT-like cell subsets in the peripheral venous blood of women with URM using flow cytometry. The expression levels of specifically related Th1 cytokines (IFN-γ and IL-2), Th2 cytokine (IL-4), and Th17 cytokines (IL-17), were measured using enzyme-linked immunosorbent assay. Results The percentage of CD16+CD56+NKT-like (Double Positive NKT-like; DPNKT-like) cell subset and the levels of IL-2 and IFN-γ were significantly elevated in the blood of non-pregnant and pregnant patients with URM compared with the healthy control groups, and these parameters were significantly increased after pregnancy in the same patients with URM. Based on the prevalence of the candidate immunological factors in patients with URM, the prognostic significance of the NKT-like cell subsets, IFN-γ, and IL-2 profiles were evaluated as potential predictors of URM. A cut-off point of 2.55% for the DPNKT-like cell subset in the blood and cut-off values of 39.5 and 20.5 pg/ml for the levels of IFN-γ and IL-2, respectively could be used for the prediction of the risk of spontaneous abortion. To the best of our knowledge, this is the first study that described the prognostic significance of the aforementioned immunological parameters before and after pregnancy, and highlighted the correlation of NKT-like cells and the candidate Th1 cytokines with pregnancy loss in women with URM. Conclusions DPNKT-like cells, IFN-γ, and IL-2 patient profiles could be used as markers to predict the risk of miscarriage in patients with URM.
dc.identifier.citationKhalaf, W. S., Mahmoud, M. R., Elkhatib, W. F., Hashem, H. R., & Soliman, W. E. (2021). Phenotypic characterization of NKT-like cells and evaluation of specifically related cytokines for the prediction of unexplained recurrent miscarriage. Heliyon, 7(11), e08409. https://doi.org/10.1016/j.heliyon.2021.e08409
dc.identifier.doi10.1016/j.heliyon.2021.e08409
dc.identifier.issn2405-8440
dc.identifier.pages8
dc.identifier.urihttps://research.gu.edu.eg/handle/123456789/305
dc.language.isoen
dc.publisherELSEVIER
dc.relation.ispartofHeliyon
dc.source.urihttps://www.cell.com/heliyon/fulltext/S2405-8440(21)02512-3?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS2405844021025123%3Fshowall%3Dtrue#secsectitle0040
dc.subjectCytokines
dc.subjectUnexplained recurrent miscarriage
dc.subjectPrediction
dc.subjectNKT-like cells
dc.titlePhenotypic characterization of NKT-like cells and evaluation of specifically related cytokines for the prediction of unexplained recurrent miscarriage
dc.typeArticle
dspace.entity.typePublication
oaire.citation.issue11
oaire.citation.volume7
relation.isAuthorOfPublication92797dd3-4a77-4ef2-b20d-bc2e3a26d692
relation.isAuthorOfPublication.latestForDiscovery92797dd3-4a77-4ef2-b20d-bc2e3a26d692
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