Publication: Biocontrol of okra-rot-causing Cochliobolus spicifer-CSN-20 using secondary metabolites of endophytic fungi associated with Solenostemma arghel
Biocontrol of okra-rot-causing Cochliobolus spicifer-CSN-20 using secondary metabolites of endophytic fungi associated with Solenostemma arghel
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Publisher DOI
10.1016/j.aoas.2022.04.001
Date
2022-04-21
Type
Article
Authors
Abdel-Motaal, Fatma F.
Kamel, Noha M.
El-Sayed, Magdi Abdel-Radi
Abou-Ellail, Mohamed
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Journal ISSN
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Publisher
Elsevier
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Source
https://www.sciencedirect.com/science/article/pii/S0570178322000033?dgcid=rss_sd_all
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Abstract
Rot disease is responsible for serious economic losses related to okra (Abelmoschus esculentus) crops cultivated in Upper Egypt. Colonies with a consistent morphology were isolated from the infected okra stems and leaves and subjected to morphological and molecular examinations. The causal pathogen was identified as Cochliobolus spicifer based on morphological fungus descriptions, as well as on the amplified 28S rDNA and internal transcribed spacer region (ITS) sequences, which showed 99%–100% similarity to the sequences of C. spicifer-CSN-20 strains. The volatile and non-volatile organic compounds (VOCs and n-VOCs, respectively) produced by the endophytic fungi that are associated with the medicinal plant Solenostemma arghel, namely Fusarium solani-F4-1007, Penicillium verrucosum-F2-1006, and Aspergillus terreus-F5-1008, inhibited the growth of the C. spicifer pathogen by 34.2%,31.4%, and 30.5%, respectively. In total,27 VOCs were identified by GC/MS, among which eight were specific to A. terreus-F5-1008, eight to P. verrucosum-F2-1006, and three to F. solani-F4-1007, whereas nine VOCs were commonly produced by the three endophytic fungi. Moreover, F. solani-F4-1007-produced VOCs and n-VOCs exhibited the highest antifungal activity, with 37.27% and 37.1% inhibition against C. spicifer colony growth, respectively. The potent antifungal VOCs produced by F. solani-F4-1007 were identified as 3,4-dihydro-2 h-1,5-(3″-t-butyl) benzodioxepine, 4-(2-hydroxyethyl) phenol, and phenylethyl alcohol using GC/MS. Therefore, F. solani-F4-1007 was tested as a potential biocontrol agent against C. spicifer-CSN-20 using an in-planta assay. Okra plants treated with endophytic F. solani-F4-1007did not show any disease symptoms, whereas those that were not treated with F. solani-F4-1007 exhibited severe disease symptoms when challenged with inoculation of the C. spicifer pathogen. Our results demonstrated the contribution of the endophytic fungus F. solani-F4-1007 as a potential biocontrol agent against the C. spicifer pathogen, to improve okra growth.
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Keywords
Abelmoschus esculentus, Cochliobolus spicifer, Endophyte, Rot disease, Solenostemma arghel, Volatiles, Non-volatiles
Citation
Abdel-Motaal, F. F., Kamel, N. M., El-Sayed, M. A., & Abou-Ellail, M. (2022). Biocontrol of okra-rot-causing Cochliobolus spicifer-CSN-20 using secondary metabolites of endophytic fungi associated with Solenostemma arghel. Annals of Agricultural Sciences, 67(1), 24-33. https://doi.org/10.1016/j.aoas.2022.04.001
Journal Title
Annals of Agricultural Sciences
Journal Title
Journal Title
Volume
67
Issue
1
Volume
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© 2023 by the authors. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.