Person:
Khafaga, Doaa S. R.

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0000-0002-0576-0100
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57443360500
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Doaa Sayed Rashwan Khafaga
Last Name
Khafaga
First Name
Doaa S. R.
Main Affiliation
Galala University
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Lecturer

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  • Publication
    Open Access
    Green Synthesis of Zinc oxide Nanocomposite Using Fusarium oxysporum and Evaluation of the Anticancer Effect on Hepatocellular Carcinoma
    (National Information and Documentation Center (NIDOC), 2022-04-01) Abd El Hamed, May Mohamed; Diaa El-deen, Mohamed; Afify, Mei Mohamed; Mohamed, Mona Hassan; Mohamed, Abd El Razik Farrag; Nagy, Rana Assem; Khafaga, Doaa S. R.
    Nanoparticles can be synthesized by chemical, physical and biological system methods. In this work, Superparamagnetic Iron Oxide@Silver nanoparticles (SPION@Ag NPs) were modified by zinc oxide nanoparticles (ZnO NPs). SPION@Ag core shell NPs were synthesized by the biological method using Fusarium oxysporum fungus and coated with ZnO NPs. This method is eco-friendly and low cost. Characterization techniques such as UV, FTIR, HR-TEM, HR-SEM, XRD and zeta potential were used. The most common type of liver cancer is hepatocellular carcinoma (HCC). Hepatocellular carcinoma was induced by a single dose of diethyl nitrosamine (DEN) 60 mg/Kg b.wt. and was followed after two days with carbon tetra chloride (CCl4) diluted with paraffin oil (50% v/v, 2 ml/Kg b.wt.) twice a week for one month. A histopathology examination was done after scarifying. Clear changes were shown in the comparison between the HCC group treated with ZnO@SPION@Ag nanocomposite and the positive control group. Liver function tests showed a highly significant decrease after using the nanocomposite for HCC treatment. Our study aim is to evaluate the therapeutic anticancer effect of ZnO@SPION@Ag nanocomposite on hepatocellular carcinoma in male albino rats, which was synthesized by a new method called green chemistry or green synthesis.
  • Publication
    Open Access
    Anticancer effect of Sorafenib-loaded iron oxide nanoparticles and bee venom on some genes expression in hepatocellular carcinoma
    (National Information and Documentation Center (NIDOC), 2022-07-18) Nagy, Rana A. A.; Mohamed, Mona; Elhakim, Heba K.A.; Abd El-Maksoud, Mohamed D.E.; Afify, Mie; Mohamed, Abd El Razik; Eid, May; Khafaga, Doaa S. R.
    Objective Superparamagnetic iron oxide@silver@chitosan core-shell nanoparticles could be a promising anticancer agent. This experiment was designed to compare bee venom, a natural substance that has been utilized as a traditional medicine for the treatment of cancer, to a new strategy in the recession of hepatocellular carcinoma utilizing Fe2O3@Ag@Cs nanoparticles. Methods: Group I is the negative control group, and Group II is the positive control group. The positive control group received a single intraperitoneal injection of 60 mg/kg b.wt. diethyl nitrosamine, followed by two days of carbon tetrachloride diluted with paraffin oil. Group III was given sorafenib twice a week by gavage for one month, Group IV was given Fe2O3@Ag@Cs core shell NPs, Group V was given Fe2O3@Ag@Cs core shell NPs loaded with sorafenib, and Group VI was given bee venom. Results: In comparison between the treated groups and the positive control group, biochemical data demonstrated a highly significant drop in ALT, AST, ALP, and AFP after treatment, as well as significant down-regulation in the DTL gene, while DUSP1, SOCS2, and NFKB1A exhibited up-regulation after treatment. Conclusion: Because there was a significant variation in the fold of expression in studied genes, which related to the degree of pathogenicity of hepatocellular carcinoma and gave good valuable insights about the progression of HCC and prognosis, loading sorafenib on Fe2O3@Ag@Cs core-shell NPs is superior to bee venom in the treatment of hepatocellular carcinoma.