Publication: Improvement of caffeic acid biotransformation into para‐hydroxybenzoic acid by CI‐24 via gamma irradiation and model‐based optimization.
Improvement of caffeic acid biotransformation into para‐hydroxybenzoic acid by CI‐24 via gamma irradiation and model‐based optimization.
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Publisher DOI
10.1002/bab.2124
Date
2021-04-19
Type
Article
Authors
Singab, Raghda Abdelnasser
Elleboudy, Nooran Sherif
Yassein, Mahmoud Abdulmegead
Hassouna, Nadia Adelhaleem
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Publisher
John Wiley & Sons
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Source
https://iubmb.onlinelibrary.wiley.com/doi/full/10.1002/bab.2124
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Abstract
Para-hydroxybenzoic acid (PHBA) has great potential in biological applications due to its putative antiviral activity against SARS-CoV-2 and its antimicrobial activity in the face of the radically increasing number of multidrug-resistant pathogens. This is in addition to its antimutagenic, anti-inflammatory, antioxidant, hypoglycemic, antiestrogenic, and antiplatelet aggregating activities. In this study, an approximate sixfold increase in the production of PHBA was achieved via biotransformation of caffeic acid by Candida albicans. The improvement was performed in two steps: first, through mutation by gamma irradiation (5 KGy dose), resulting in the recovery of a mutant (CI-24), which produced approximately triple the amount of PHBA produced by the wild-type isolate. Then, biotransformation by this mutant was further optimized via response surface methodology model-based optimization. The maximum PHBA production (7.47 mg/mL) was obtained in a fermentation medium composed of 1% w/v yeast extract as a nitrogen source, with an initial pH of 6.6, incubated at 28 °C at an agitation rate of 250 rpm. To further enhance the performance and economics of the process, cells of the CI-24 mutant were immobilized in calcium alginate beads and could retain an equivalent biotransformation capacity after three successive biotransformation cycles.
Description
Keywords
Response surface methodology, Mutation, Optimization, Candida albicans, Caffeic acid, Biotransformation
Citation
Singab, R. A., Elleboudy, N. S., Elkhatib, W. F., Yassein, M. A., & Hassouna, N. A. (2021, March 7). Improvement of caffeic acid biotransformation into para‐hydroxybenzoic acid by Candida albicans CI‐24 via gamma irradiation and model‐based optimization. Biotechnology and Applied Biochemistry, 69(2), 469–478. https://doi.org/10.1002/bab.2124
Journal Title
Biotechnology and Applied Biochemistry
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Volume
69
Issue
2
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© 2023 by the authors. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.