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Improvement of caffeic acid biotransformation into para‐hydroxybenzoic acid by CI‐24 via gamma irradiation and model‐based optimization.

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dc.contributor.authorSingab, Raghda Abdelnasser
dc.contributor.authorElleboudy, Nooran Sherif
dc.contributor.authorYassein, Mahmoud Abdulmegead
dc.contributor.authorHassouna, Nadia Adelhaleem
dc.contributor.authorElkhatib, Walid F.
dc.date.accessioned2022-09-22T08:36:19Z
dc.date.available2022-09-22T08:36:19Z
dc.date.issued2021-04-19
dc.description.abstractPara-hydroxybenzoic acid (PHBA) has great potential in biological applications due to its putative antiviral activity against SARS-CoV-2 and its antimicrobial activity in the face of the radically increasing number of multidrug-resistant pathogens. This is in addition to its antimutagenic, anti-inflammatory, antioxidant, hypoglycemic, antiestrogenic, and antiplatelet aggregating activities. In this study, an approximate sixfold increase in the production of PHBA was achieved via biotransformation of caffeic acid by Candida albicans. The improvement was performed in two steps: first, through mutation by gamma irradiation (5 KGy dose), resulting in the recovery of a mutant (CI-24), which produced approximately triple the amount of PHBA produced by the wild-type isolate. Then, biotransformation by this mutant was further optimized via response surface methodology model-based optimization. The maximum PHBA production (7.47 mg/mL) was obtained in a fermentation medium composed of 1% w/v yeast extract as a nitrogen source, with an initial pH of 6.6, incubated at 28 °C at an agitation rate of 250 rpm. To further enhance the performance and economics of the process, cells of the CI-24 mutant were immobilized in calcium alginate beads and could retain an equivalent biotransformation capacity after three successive biotransformation cycles.
dc.identifier.citationSingab, R. A., Elleboudy, N. S., Elkhatib, W. F., Yassein, M. A., & Hassouna, N. A. (2021, March 7). Improvement of caffeic acid biotransformation into para‐hydroxybenzoic acid by Candida albicans CI‐24 via gamma irradiation and model‐based optimization. Biotechnology and Applied Biochemistry, 69(2), 469–478. https://doi.org/10.1002/bab.2124
dc.identifier.doi10.1002/bab.2124
dc.identifier.issn0885-4513
dc.identifier.issn1470-8744
dc.identifier.pagesPages 469-478
dc.identifier.urihttps://research.gu.edu.eg/handle/123456789/118
dc.language.isoen
dc.publisherJohn Wiley & Sons
dc.relation.ispartofBiotechnology and Applied Biochemistry
dc.source.urihttps://iubmb.onlinelibrary.wiley.com/doi/full/10.1002/bab.2124
dc.subjectResponse surface methodology
dc.subjectMutation
dc.subjectOptimization
dc.subjectCandida albicans
dc.subjectCaffeic acid
dc.subjectBiotransformation
dc.titleImprovement of caffeic acid biotransformation into para‐hydroxybenzoic acid by CI‐24 via gamma irradiation and model‐based optimization.
dc.typeArticle
dspace.entity.typePublication
oaire.citation.issue2
oaire.citation.volume69
relation.isAuthorOfPublication92797dd3-4a77-4ef2-b20d-bc2e3a26d692
relation.isAuthorOfPublication.latestForDiscovery92797dd3-4a77-4ef2-b20d-bc2e3a26d692
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