Publication: Defective cytokinin signaling reprograms lipid and flavonoid gene-to-metabolite networks to mitigate high salinity in Arabidopsis
Defective cytokinin signaling reprograms lipid and flavonoid gene-to-metabolite networks to mitigate high salinity in Arabidopsis
Repository URI
Publisher DOI
10.1073/pnas.2105021118
Date
2021
Type
Article
Authors
Abdelrahman, Mostafa
Nishiyama, Rie
Tran, Cuong Duy
Kusano, Miyako
Nakabayashi, Ryo
Okazaki, Yozo
Matsuda, Fumio
Montes, Ricardo A. Chávez
Mostofa, Mohammad Golam
Li, Weiqiang
Journal Title
Journal ISSN
Volume Title
Publisher
National Academy of Science
Publisher DOI
Source
https://www.pnas.org/doi/full/10.1073/pnas.2105021118#executive-summary-abstract
Research Projects
Organizational Units
Journal Issue
Abstract
Cytokinin (CK) in plants regulates both developmental processes and adaptation to environmental stresses. Arabidopsis histidine phosphotransfer ahp2,3,5 and type-B Arabidopsis response regulator arr1,10,12 triple mutants are almost completely defective in CK signaling, and the ahp2,3,5 mutant was reported to be salt tolerant. Here, we demonstrate that the arr1,10,12 mutant is also more tolerant to salt stress than wild-type (WT) plants. A comprehensive metabolite profiling coupled with transcriptome analysis of the ahp2,3,5 and arr1,10,12 mutants was conducted to elucidate the salt tolerance mechanisms mediated by CK signaling. Numerous primary (e.g., sugars, amino acids, and lipids) and secondary (e.g., flavonoids and sterols) metabolites accumulated in these mutants under nonsaline and saline conditions, suggesting that both prestress and poststress accumulations of stress-related metabolites contribute to improved salt tolerance in CK-signaling mutants. Specifically, the levels of sugars (e.g., trehalose and galactinol), amino acids (e.g., branched-chain amino acids and γ-aminobutyric acid), anthocyanins, sterols, and unsaturated triacylglycerols were higher in the mutant plants than in WT plants. Notably, the reprograming of flavonoid and lipid pools was highly coordinated and concomitant with the changes in transcriptional levels, indicating that these metabolic pathways are transcriptionally regulated by CK signaling. The discovery of the regulatory role of CK signaling on membrane lipid reprogramming provides a greater understanding of CK-mediated salt tolerance in plants. This knowledge will contribute to the development of salt-tolerant crops with the ability to withstand salinity as a key driver to ensure global food security in the era of climate crisis.
Description
Keywords
comparative metabolomics, cytokinin signaling, salt stress, regulatory network, comparative transcriptomics
Citation
Abdelrahman, M., Nishiyama, R., Tran, C. D., Kusano, M., Nakabayashi, R., Okazaki, Y., Matsuda, F., Chávez Montes, R. A., Mostofa, M. G., Li, W., Watanabe, Y., Fukushima, A., Tanaka, M., Seki, M., Saito, K., Herrera-Estrella, L., & Tran, L. S. P. (2021). Defective cytokinin signaling reprograms lipid and flavonoid gene-to-metabolite networks to mitigate high salinity in Arabidopsis. Proceedings of the National Academy of Sciences, 118(48). https://doi.org/10.1073/pnas.2105021118
Journal Title
Proceedings of the National Academy of Sciences
Journal Title
Journal Title
Volume
118
Issue
48
Volume
Collections
© 2023 by the authors. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.